UBC32 Antibody

Code CSB-PA881801XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) UBC32 Polyclonal antibody
Uniprot No.
Target Names
UBC32
Alternative Names
UBC32; At3g17000; K14A17.7; Ubiquitin-conjugating enzyme E2 32; E2 ubiquitin-conjugating enzyme 32; Ubiquitin carrier protein 32
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana UBC32 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.
Gene References into Functions
  1. UBC32 negatively controls the stability of AtOS9, a component in the HRD1 complex. PMID: 28040559
  2. The plant ubiquitin-conjugating enzyme 32 (UBC32), an ER-bound E2 working in the DOA10 complex, is maintained at low levels under standard conditions by proteasome-dependent degradation mediated by the HRD1 complex, the other E3 complex involved in ERAD. PMID: 27322605
  3. UBC32 mediated oxidative tolerance. PMID: 22884097
  4. Data supported the contention that UBC32 plays a role in the BR-mediated salt stress response and that BR signaling is necessary for the plant to tolerate salt. PMID: 22214659
Subcellular Location
Membrane; Single-pass membrane protein.
Protein Families
Ubiquitin-conjugating enzyme family
Database Links

KEGG: ath:AT3G17000

STRING: 3702.AT3G17000.1

UniGene: At.6347

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