Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) UBC7 Polyclonal antibody

Uniprot No.

Q02159

Target Names

UBC7

Alternative Names

UBC7 antibody; QRI8 antibody; YMR022W antibody; YM9711.12Ubiquitin-conjugating enzyme E2 7 antibody; EC 2.3.2.23 antibody; E2 ubiquitin-conjugating enzyme 7 antibody; Ubiquitin carrier protein antibody; Ubiquitin-conjugating enzyme E2-18 kDa antibody; Ubiquitin-protein ligase antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) UBC7 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains, and of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). Involved in resistance to cadmium poisoning.

Gene References into Functions

Cue1 positions Ubc7 and the distal acceptor ubiquitin for rapid polyubiquitination. PMID: 27264873
Data indicate the crystal structure of the Ubc7p-binding region (U7BR) of Cue1p with Ubc7p. PMID: 23665230
reveal a previously unobserved stimulation of Ubc7p E2 activity by Cue1p PMID: 18321851
Data show that a discrete C-terminal Ubc7p binding region of Cue1p is required for ER-associated degradation and for Ubc7p-dependent ubiquitylation by Hrd1p in vitro. PMID: 19366730

Subcellular Location

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=Anchored via the membrane protein CUE1 to the endoplasmic reticulum membrane.

Protein Families

Ubiquitin-conjugating enzyme family

Database Links

KEGG: sce:YMR022W

STRING: 4932.YMR022W

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UBC7 Antibody

Product Details

Customer Reviews and Q&A

Target Background