UBC9 Antibody

Code CSB-PA025457XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) UBC9 Polyclonal antibody
Uniprot No.
Target Names
UBC9
Alternative Names
UBC9 antibody; YDL064W antibody; SUMO-conjugating enzyme UBC9 antibody; EC 2.3.2.- antibody; RING-type E3 SUMO transferase UBC9 antibody; Ubiquitin carrier protein 9 antibody; Ubiquitin-conjugating enzyme E2-18 kDa antibody; Ubiquitin-protein ligase antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) UBC9 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2).
Gene References into Functions
  1. Ubc9 sumoylation is a key event for meiotic synaptonemal complex formation. SUMO Ubc9 enhances catalysis by binding and orienting donor SUMO charged Ubc9. PMID: 23644018
  2. These findings establish a functional interaction between N-terminal and substrate-binding domains of Ubc9 and distinguish the activities of E3 ligases Siz1 and Siz2 in regulating cellular responses to genotoxic stress. PMID: 16782883
  3. analysis of a SUMO-binding-motif mimic bound to Smt3p-Ubc9p PMID: 17475278
  4. The crystal structure of sumoylated Ubc9 demonstrates how the newly created binding interface can provide a gain in affinity otherwise provided by E3 ligases. PMID: 18691969
Subcellular Location
Nucleus.
Protein Families
Ubiquitin-conjugating enzyme family
Database Links

KEGG: sce:YDL064W

STRING: 4932.YDL064W

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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