eff Antibody

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Code	CSB-PA340739XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) eff Polyclonal antibody

Uniprot No.

P25867

Target Names

eff

Alternative Names

eff antibody; UbcD1 antibody; CG7425 antibody; Ubiquitin-conjugating enzyme E2-17 kDa antibody; EC 2.3.2.23 antibody; E2 ubiquitin-conjugating enzyme D1 antibody; Protein effete antibody; Ubiquitin carrier protein antibody; Ubiquitin-protein ligase antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) eff protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Required for proper telomere behavior during cell divisions and possibly for ubiquitination of proteins involved in postmeiotic stages of spermatogenesis. Deletion mutations are lethal in homozygotes.

Gene References into Functions

UbcD1 regulates Hedgehog signaling by directly modulating Ci ubiquitination and processing. PMID: 28887318
eff regulates both telomere position effect and position effect variegation. PMID: 23821599
the UbcD1 substrate(s) binds chromosomal termini in a sequence-independent manner PMID: 12723710

Protein Families

Ubiquitin-conjugating enzyme family

Database Links

KEGG: dme:Dmel_CG7425

STRING: 7227.FBpp0082477

UniGene: Dm.4048

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eff Antibody

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Target Background