Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans ufd-2 Polyclonal antibody

Uniprot No.

Q09349

Target Names

ufd-2

Alternative Names

ufd-2 antibody; T05H10.5 antibody; Ubiquitin conjugation factor E4 ufd-2 antibody; EC 2.3.2.27 antibody; E4 ubiquitin-protein ligase ufd-2 antibody; RING-type E3 ubiquitin transferase E4 antibody; Ubiquitin fusion degradation protein 2 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans ufd-2 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Acts as an E4 ubiquitin ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. The elongation of preexisting ubiquitin chains preferentially targets ubiquitin 'Lys-29' and 'Lys-48' residues. Also functions as an E3 ligase in conjunction with specific E1 and E2 ligases. Probably by regulating protein ubiquitination at DNA damage repair sites, coordinates DNA double-strand-break repair and apoptosis in the germline. Required for germline apoptosis in response to DNA damage downstream of cep-1. Involved in the resolution of DNA-repair sites by promoting the release of rad-51 from DNA damage foci. In association with protein-ligase chn-1, acts as an E3/E4 ligase to poly-ubiquitinate lysine residues in the UCS domain of myosin chaperone unc-45. By targeting myosin chaperone unc-45 for proteasomal degradation, regulates myosin assembly in body wall muscles in association with cdc-48.1 and chn-1. However, in a contrasting study, acts as an E3 ligase, independently of chn-1, to poly-ubiquitinate unc-45 without promoting unc-45 proteasomal degradation. Instead, uses unc-45 as an adapter protein to recruit and poly-ubiquitinate unfolded myosin heavy chain B unc-54.

Gene References into Functions

UFD-2 is not regulating the protein levels of UNC-45 in muscle cells, but rather shows the characteristic properties of a bona fide E3 ligase involved in protein quality control. UFD-2 preferentially targets unfolded protein segments. PMID: 29396393
Ligase activity of UFD-2 triggers DNA double-strand breaks -induced apoptosis. UFD-2 supports RAD-51 dissociation from DNA-repair sites. PMID: 27669035

Subcellular Location

Cytoplasm. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nucleolus.

Protein Families

Ubiquitin conjugation factor E4 family

Tissue Specificity

Expressed in the germline (at protein level).

Database Links

STRING: 6239.T05H10.5b

UniGene: Cel.7502

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ufd-2 Antibody

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