UBP3 Antibody

Code CSB-PA982697XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) UBP3 Polyclonal antibody
Uniprot No.
Target Names
UBP3
Alternative Names
UBP3 antibody; YER151C antibody; Ubiquitin carboxyl-terminal hydrolase 3 antibody; EC 3.4.19.12 antibody; Deubiquitinating enzyme 3 antibody; Ubiquitin thioesterase 3 antibody; Ubiquitin-specific-processing protease 3 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) UBP3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Has an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety in natural or engineered linear fusion proteins, irrespective of their size or the presence of an N-terminal extension to ubiquitin. Plays a role in regulation of silencing by interacting with SIR4. Also, in conjunction with BRE5, cleaves ubiquitin, leading to the subsequent mono-ubiquitination of SEC23. Required for ribophagy, a process which relocalizes ribosomal particles into the vacuole for degradation in response to starvation.
Gene References into Functions
  1. Ubp3 is essential for proper proteasome function in euploid cells, and deletion of this deubiquitinase leads to further proteasome-mediated proteotoxicity in aneuploid yeast PMID: 27807036
  2. Ubp3 deubiquitinating protease is required for efficient stress granule assembly in Saccharomyces cerevisiae PMID: 26503781
  3. these results suggest that the autophagy and proteasomal pathways degrade distinct translation and RNA turnover factors in a Ubp3p-dependent manner during nitrogen starvation. PMID: 25795416
  4. Ubp3 acts as an antisilencing factor at all three loci that display silencing in S. cerevisiae. PMID: 24760971
  5. Ubp3 overproduction counteracts deficiency of Hsp70s by the removal of damaged proteins deposited in inclusion bodies (JUNQ) during both aging and heat stress. PMID: 24596250
  6. Ubp3 is a new regulator of the Ras/PKA signaling pathway and suggest that Ubp3 regulates this pathway by controlling the ubiquitination of Ras GTPase-accelerating protein Ira2 PMID: 23476013
  7. Disruption of UBP3 leads to enhanced UV resistance and elevated Rad4 stability. PMID: 20876584
  8. Results suggest that blm3-1 is a nonsense mutation in the ubiquitin protease gene UBP3. PMID: 16997324
  9. Data show that Ubp3 deubiquitylates RNA polymerase II in yeast. PMID: 18498751

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Protein Families
Peptidase C19 family
Database Links

KEGG: sce:YER151C

STRING: 4932.YER151C

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