DOA4 Antibody

Code CSB-PA025731XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DOA4 Polyclonal antibody
Uniprot No.
Target Names
DOA4
Alternative Names
DOA4 antibody; DOS1 antibody; MUT4 antibody; NPI2 antibody; SSV7 antibody; UBP4 antibody; YDR069C antibody; D4270 antibody; YD8554.02C antibody; YD9609.23C antibody; Ubiquitin carboxyl-terminal hydrolase 4 antibody; EC 3.4.19.12 antibody; Deubiquitinating enzyme 4 antibody; Ubiquitin thioesterase 4 antibody; Ubiquitin-specific-processing protease 4 antibody; Vacuole biogenesis protein SSV7 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DOA4 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Involved in the ammonium-induced down-regulation of the GAP1 permease and the UME3 destruction in response to oxidative stress. Has a role in the RAD9 checkpoint response to TOP1 poisons. Required for promoting coordination of DNA replication and avoids DNA overreplication.
Gene References into Functions
  1. Doa4 physically interacts with the ESCRT-III component Snf7 and preferentially cleaves Lys63-linked ubiquitin oligomers involved in membrane protein trafficking PMID: 26427873
  2. Binding of Vps20 to the Doa4 N-terminus inhibits a non-catalytic function of Doa4 that promotes ILV formation. PMID: 23444383
  3. This is the first demonstration of a physiologically important DUB (Doa4) subcellular localization signal and provides a striking example of the functional diversification of DUB paralogues by the evolution of alternative spatial signals. PMID: 17145966
  4. Deubiquitination by Doa4 of cargo proteins and/or some components of the multivesicular body (MVB) sorting machinery is essential to correct sorting of cargoes into the MVB pathway. PMID: 17376168
  5. Dual mechanisms specify Doa4-mediated deubiquitination at multivesicular bodies. PMID: 17446860

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Subcellular Location
Cytoplasm. Late endosome membrane; Peripheral membrane protein. Note=Recruited to the late endosome by BRO1.
Protein Families
Peptidase C19 family
Database Links

KEGG: sce:YDR069C

STRING: 4932.YDR069C

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