Usp47 Antibody

Code CSB-PA636559XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Usp47 Polyclonal antibody
Uniprot No.
Target Names
Usp47
Alternative Names
Usp47 antibody; Ubp64E antibody; CG5486Ubiquitin carboxyl-terminal hydrolase 47 antibody; EC 3.4.19.12 antibody; Ubiquitin thioesterase 47 antibody; Ubiquitin-specific-processing protease 47 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Usp47 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ubiquitin-specific protease that deubiquitinates target proteins to regulate different cellular and developmental pathways. Functions downstream of Dsor1/MEK to positively regulate the Ras/MAPK signaling pathway. Likely to modulate the pathway during various cellular and developmental processes including rl/MAPK activation by the receptors InR, Egfr and sevenless/sev. Functions in the post-translational stabilization of rl/MAPK levels in a mechanism that is independent of rl activity and opposes the activity of the E2 enzyme Unc6 and the putative E3 ligases poe, Ufd4 and Kcmf1, which mediate the ubiquitination and proteasomal degradation of rl. During eye development it may also act downstream of rl/MAPK to negatively regulate the Ras/MAPK signaling pathway by stabilizing the transcriptional repressor ttk and consequently inhibiting photoreceptor cell development. This suggests that at least during eye development, it may act in both the positive and negative regulation of the Ras/MAPK signaling pathway to mediate the development of different cell types. Positively regulates border follicle cell migration during oogenesis by mediating the deubiquitination and stabilization of slbo. In the wing disks it positively regulates wg signaling by stabilizing arm. Has an effect on position-effect variegation.
Gene References into Functions
  1. In the presence of USP47, MAPK would be stabilized as USP47 acts to deubiquitinate MAPK, thus counteracting the direct ubiquitination of MAPK by POE/UBR4 activity. PMID: 27552662
Subcellular Location
Nucleus.
Protein Families
Peptidase C19 family
Database Links

KEGG: dme:Dmel_CG5486

STRING: 7227.FBpp0076802

UniGene: Dm.3704

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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