Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Ubr3 Polyclonal antibody

Uniprot No.

Q9W3M3

Target Names

Ubr3

Alternative Names

Ubr3 antibody; CG42593 antibody; E3 ubiquitin-protein ligase Ubr3 antibody; EC 2.3.2.27 antibody; E3 ubiquitin-protein transferase Ubr3 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Ubr3 protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues, leading to their ubiquitination and subsequent degradation. Binds to the E3 ubiquitin-protein ligase Diap1 and enhances its ubiquitination and anti-apoptotic functions. Essential during trichome development for the ubiquitination of the N-terminus of ovo isoform B (svb), converting it from a transcriptional inhibitor to an activator. Positively regulates a hh-signaling pathway which functions in photoreceptor differentiation. Activation of hh up-regulates transcription of Ubr3, which in turn promotes hh signaling by mediating the ubiquitination and degradation of cos. Necessary for auditory transduction: plays a role in Johnston's organ organization by acting in the regulation of zip and ck function in scolopidial apical attachment. Likely to function by acting in a pathway that negatively regulates the ubiquitination of zip, consequently affecting its interaction with ck. May also negatively regulate a component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex Cul1, which also appears to function in the negative regulation of the zip-ck interaction and scolopidial apical attachment.

Gene References into Functions

Ubr3 negatively regulates the mono-ubiquitination of non-muscle Myosin II, a protein associated with hearing loss in humans. PMID: 27331610
We identified Ubr3, an E3 ubiquitin ligase, as a novel, positive regulator of Hh signaling in Drosophila and vertebrates. Hh signaling regulates the Ubr3-mediated poly-ubiquitination and degradation of Cos2, a central component of Hh signaling. In developing Drosophila eye discs, loss of ubr3 leads to a delayed differentiation of photoreceptors and a reduction in Hh signaling. PMID: 27195754
Ubr3 interacts with DIAP1 and positively regulates DIAP1 activity, possibly by maintaining its active conformation in the apoptosis pathway. PMID: 25146930

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

UBR1 family

Database Links

KEGG: dme:Dmel_CG42593

STRING: 7227.FBpp0303730

UniGene: Dm.18600

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Ubr3 Antibody

Product Details

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Target Background