APP-BP1 Antibody

Code CSB-PA893801XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) APP-BP1 Polyclonal antibody
Uniprot No.
Target Names
APP-BP1
Alternative Names
APP-BP1 antibody; CG7828 antibody; Nedd8-activating enzyme E1 regulatory subunit antibody; Beta-amyloid precursor binding protein 1 antibody; dAPP-BP1 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) APP-BP1 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Regulatory subunit of the dimeric Uba3-APP-BP1 E1 enzyme. E1 activates Nedd8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a Nedd8-Uba3 thioester and free AMP. E1 finally transfers Nedd8 to the catalytic cysteine of UbcE2M. Required for Cul1 and Cul3 neddylation. Appl and APP-BP1 interact antagonistically during development.
Protein Families
Ubiquitin-activating E1 family, ULA1 subfamily
Database Links

KEGG: dme:Dmel_CG7828

STRING: 7227.FBpp0075938

UniGene: Dm.11576

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