unc-18 Antibody

Code CSB-PA271009XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans unc-18 Polyclonal antibody
Uniprot No.
Target Names
unc-18
Alternative Names
unc-18 antibody; lan-2 antibody; F27D9.1 antibody; Putative acetylcholine regulator unc-18 antibody; Uncoordinated protein 18 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans unc-18 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
May participate in the axonal transport system and influences the acetylcholine flow in motor neurons. Probably by regulating neuronal transmission downstream of lin-3 and receptor lin-23 and phospholipase plc-3 and upstream of innexin unc-7 and egl-4/PKG in ALA neurons, involved in the decrease in pharyngeal pumping during the quiescent state that precedes each larval molt.
Gene References into Functions
  1. data demonstrate that an individual DAG-dependent thermosensory behavior of an organism is effected specifically by the downstream PKC-2 phosphorylation of UNC-18 on Ser322 in AFD neurons. PMID: 22593072
  2. data implicate a novel role for an exposed loop in domain 3b of Munc18-1 in transducing regulation of vesicle fusion independent of closed-conformation syntaxin binding PMID: 21445306
  3. A reduction of docked synaptic vesicles at the active zone occurs in UNC-18 mutants, suggesting that UNC-18 functions, directly or indirectly, as a facilitator of vesicle docking. PMID: 12973353
  4. UNC-18 acts as a molecular chaperone for Syntaxin transport in neurons PMID: 18596236
  5. Results suggest that ethanol acts, at least partially, at the level of exocytotic vesicle fusion and that its acute effects are ameliorated by point mutations in UNC-18. PMID: 18923141
  6. binding of UNC-18 to closed syntaxin is dispensable for membrane fusion, whereas interaction with the syntaxin N-terminus is essential for neuronal exocytosis in vivo PMID: 19032153

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Protein Families
STXBP/unc-18/SEC1 family
Tissue Specificity
Motor neurons and a limited number of neurons constituting nerve ganglia.
Database Links

KEGG: cel:CELE_F27D9.1

STRING: 6239.F27D9.1c

UniGene: Cel.5760

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