unc-5 Antibody

Code CSB-PA634609XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans unc-5 Polyclonal antibody
Uniprot No.
Target Names
unc-5
Alternative Names
unc-5 antibody; B0273.4Netrin receptor unc-5 antibody; Uncoordinated protein 5 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans unc-5 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Receptor for netrin (unc-6) required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon migration is mediated by the secreted unc-6, which promotes attraction of neurons and axons through binding to the unc-40 receptor, while repulsion requires both unc-5 and unc-40 receptors. Involved in the ventral-dorsal and anterior-posterior migration of distal tip cells along the body, which may be mediated by Wnt receptor mom-5, ced-10/Rac, ced-12/ELMO and mig-2/RhoG.
Gene References into Functions
  1. Unc5 requires both Ig domains to interact with netrin. PMID: 15574733
  2. UNC-51 and UNC-14 regulate the subcellular localization of the Netrin receptor UNC-5, and that UNC-5 uses a unique mechanism for its localization PMID: 16887826
  3. the axon guidance cue UNC-6/netrin and its receptor UNC-5 act throughout development to exclude synaptic vesicle and active zone proteins from the dendrite of the Caenorhabditis elegans motor neuron DA9, which is proximal to a source of UNC-6/netrin PMID: 18776887
  4. UNC-129, graded opposite to UNC-6/netrin, functions independent of the canonical TGF-beta receptors to regulate UNC-5 cellular responses PMID: 19169249
  5. The enhancement of unc-5 caused by an sdn-1 mutation was rescued by expression of wild-type sdn-1 in the hypodermis or nervous system rather than the distal tip cell (DTC) PMID: 19631636

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Subcellular Location
Cell membrane; Single-pass type I membrane protein. Membrane raft. Cell projection, neuron projection.
Protein Families
Unc-5 family
Tissue Specificity
Expressed in cell bodies and axons of the VNC motor neurons that extend axons to the dorsal midline and within the ventral nerve cord. Expressed in gonadal distal tip cells (DTC).
Database Links

STRING: 6239.B0273.4a

UniGene: Cel.18168

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