unc-87 Antibody

Code CSB-PA330775XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans unc-87 Polyclonal antibody
Uniprot No.
Target Names
unc-87
Alternative Names
unc-87 antibody; F08B6.4Protein unc-87 antibody; Uncoordinated protein 87 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans unc-87 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Thin filament-associated protein that is implicated in actin bundling and actin filament dynamics. Exhibits F-actin cross-linking activity. Required for the maintenance of sarcomeric actin organization in striated muscles. Competes with unc-60 isoform b for actin binding and protects actin filaments from depolymerization by unc-60, thereby contributing to actin filament stability. Cooperates with myosin to form actomyosin bundles and inhibits actomyosin ATPase activity and actomyosin motility. Might protect the myofilaments from mechanical stress.; Acts as a negative regulator of myosin-dependent contractility of smooth muscle-like cells in the somatic gonad.
Gene References into Functions
  1. Both UNC-87 isoforms bind to actin filaments and myosin to induce ATP-resistant actomyosin bundles and inhibit actomyosin motility. PMID: 25717181
  2. Data show that UNC-87 competes with ADF/cofilin (unc-60) for binding to actin filaments and inhibits ADF/cofilin-dependent filament severing and depolymerization in vitro. PMID: 17684058
Subcellular Location
Cytoplasm, myofibril, sarcomere, I band.
Protein Families
Calponin family
Tissue Specificity
Expressed in the body wall muscles. Isoform a: Expression in the pharynx, anal depressor muscle, uterine muscle, vulva and unidentified neurons in the head and the ventral region. Isoform b: Expression in the body wall muscles, spermatheca, vulva and in t
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