Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) UPS1 Polyclonal antibody

Uniprot No.

Q05776

Target Names

UPS1

Alternative Names

UPS1 antibody; YLR193C antibody; Protein UPS1 antibody; mitochondrial antibody; Unprocessed MGM1 protein 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) UPS1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Required for maintenance of normal mitochondrial morphology. Required for PCP1-dependent processing of MGM1. The UPS1:MDM35 complex mediates the transfer of phosphatidic acid (PA) between liposomes and probably functions as a PA transporter across the mitochondrion intermembrane space. Phosphatidic acid release requires dissociation of the UPS1:MDM35 complex. Phosphatidic acid import is required for cardiolipin (CL) synthesis in the mitochondrial inner membrane. With UPS2, controls the level of cardiolipin in mitochondria. Cardiolipin is a unique phospholipid with four fatty acid chains and is present mainly in the mitochondrial inner membrane where it stabilizes the electron transport chain supercomplex between complexes III and IV through direct interaction of their subunits.

Gene References into Functions

crystal structure of Ups1-Mdm35 bound with a phosphatidic acid (PA) molecule at 2.0 A resolution; study reveals molecular basis of Ups1-Mdm35-mediated PA binding and transfer and sheds new light on mechanism of intramitochondrial lipid transport by MSF1/PRELI family proteins PMID: 26071601
The Ups1-Mdm35 complex constitutes a single domain that has a deep pocket and flexible Omega-loop lid. A basic residue at the pocket bottom and the Omega-loop lid are important for phosphatidic acid extraction from the membrane following Ups1 binding. PMID: 26235513
study identified Ups1 as a lipid transfer protein that can shuttle phosphatidic acid between mitochondrial membranes; lipid transfer required the dynamic assembly of Ups1 with Mdm35 and allowed conversion of phosphatidic acid to cardiolipin in the inner membrane PMID: 23042293
The UPS1 protein is a novel mitochondrial protein required for the unique processing of Mgm1p and for normal mitochondrial shape. PMID: 16754953
deletion strains, ylr193cDelta/ylr193cDelta, yor305wDelta/yor305wDelta, and ypr170cDelta/ypr170cDelta, mate at very low frequencies with haploids of either or both MATa/MATalpha mating types. PMID: 17995956

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Subcellular Location

Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Mitochondrion intermembrane space.

Protein Families

Slowmo family

Database Links

KEGG: sce:YLR193C

STRING: 4932.YLR193C

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UPS1 Antibody

Product Details

Customer Reviews and Q&A

Target Background