URT1 Antibody

Code CSB-PA906493XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) URT1 Polyclonal antibody
Uniprot No.
Target Names
URT1
Alternative Names
URT1 antibody; At2g45620 antibody; F17K2.15 antibody; UTP:RNA uridylyltransferase 1 antibody; EC 2.7.7.52 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana URT1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
UTP:RNA uridylyltransferase with a marked preference for uridine polymerization and a distributive activity for the first added nucleotides. Uridylates oligo(A)-tailed mRNAs to prevent 3' to 5' ribonucleotytic attacks. Can prevent the 3' trimming of mRNAs still engaged on polysomes. Acts synergistically with HESO1 in unmethylated miRNA uridylation, leading to their degradation. URT1 and HESO1 prefer substrates with different 3' end nucleotides and act cooperatively to tail different forms of the same miRNAs. URT1 and HESO1 act sequentially, with URT1 mono-uridylating the miRNAs followed by their further uridylation by HESO1. Has no effect on uridylation of heterochromatic siRNAs. Able to act on AGO1-bound miRNAs and the uridylated species stay associated with AGO1.
Gene References into Functions
  1. URT1 uridylates deadenylated mRNAs and prevents excessive deadenylation. PMID: 23748567
  2. Results uncover a role for uridylation and PABP in repairing mRNA deadenylated ends and reveal that uridylation plays diverse roles in eukaryotic mRNA metabolism. PMID: 26972004
  3. UTP: RNA uridylyltransferase (URT1) is a functional paralog of HESO1. URT1 interacts with AGO1 and plays a predominant role in miRNA uridylation when HESO1 is absent. [URT1] PMID: 25928341
  4. URT1 and HESO1 prefer substrates with different 3' end nucleotides in vitro and act cooperatively to tail different forms of the same miRNAs in vivo. Moreover, both HESO1 and URT1 exhibit nucleotidyl transferase activity on AGO1-bound miRNAs. [URT1] PMID: 25928405
Subcellular Location
Cytoplasm. Cytoplasm, P-body.
Protein Families
DNA polymerase type-B-like family
Database Links

KEGG: ath:AT2G45620

STRING: 3702.AT2G45620.1

UniGene: At.19458

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