UVR8 Antibody

Code CSB-PA267275XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) UVR8 Polyclonal antibody
Uniprot No.
Target Names
UVR8
Alternative Names
UVR8 antibody; At5g63860 antibody; MGI19.7Ultraviolet-B receptor UVR8 antibody; Protein UV-B RESISTANCE 8 antibody; RCC1 domain-containing protein UVR8 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana UVR8 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
UV-B specific signaling component that acts as UV-B photoreceptor and plays a key role in establishing UV-protective responses in plants. Upon UV-B irradiation, UVR8 undergoes an immediate switch from homodimer to monomer, accumulates in the nucleus, interacts with the photomorphogenic repressor COP1 and regulates the expression of the transcription factor HY5 by associating with chromatin (through histone H2B binding) in the HY5 promoter region. UVR8 is involved in controlling aspects of leaf growth and morphogenesis in response to UV-B, is required for normal progression of endocycle and has a regulatory role in stomatal differentiation. Is required for plant circadian clock response to photomorphogenic UV-B light, partly through the transcriptional activation of responsive clock genes. Promotes photosynthetic efficiency at elevated levels of UV-B. Plays a role in mediating the effects of UV-B radiation on pathogen resistance by controlling the expression of the sinapate biosynthetic pathway. The two tryptophans, Trp-285 and Trp-233, serve collectively as the UV-B chromophore.
Gene References into Functions
  1. dimer/monomer photo-equilibrium regulated by UV-B and also influenced by temperature PMID: 26864532
  2. Tissue-autonomous and simultaneous UVR8 signalling in different tissues mediates, at least partly, developmental and defence responses to Ultraviolet B radiation. PMID: 28058744
  3. Study shows that UVR8 regulates a specific chromatin modification associated with transcriptional regulation of a set of UVR8-target genes. PMID: 27534420
  4. the cellular activity of UVR8 likely does not involve its specific binding to chromatin at target genes PMID: 26864020
  5. The results indicate that tryptophan residues at the dimer interface engage in photoinduced proton coupled electron transfer reactions that induce monomerization. PMID: 25955727
  6. role of tryptophans in the UV-B absorption of a UVR8 photoreceptor PMID: 25811850
  7. not involved in short-term responses to UV-B stress PMID: 26039469
  8. UVR8 might have an important role for integrating plant growth and stress signals PMID: 24413416
  9. Results indicate that the plant perception of UV-B is mediated by UVR8 as a UV-B-specific photoreceptor, and they support a tryptophan-based perception mechanism, with tryptophan-285 as a key residue. [UVR8] PMID: 21454788
  10. The participation of the UVR8 pathway and its interaction with nitric oxide in ultraviolet-B-induced stomatal movements in Arabidopsis, was investigated. PMID: 24586043
  11. UV-B, detected by UVR8, provides an unambiguous sunlight signal that inhibits shade avoidance responses by antagonizing the phytohormones auxin and gibberellin. PMID: 25071218
  12. suggested mechanism for the UV-B-driven dissociation of the dimer that rests on the prominent players Trp233 and Trp285 explains the experimental results obtained from mutagenesis of UVR8 PMID: 24639509
  13. molecular dynamics simulations in conjunction with umbrella sampling were used to calculate the binding free energy for the wild type UVR8 dimer and three of its mutants PMID: 23745796
  14. Three tryptophans in the beta-propeller core are important in maintaining structural stability and function of UVR8. PMID: 23012433
  15. Data indicate that UVR8-dependent UV-B acclimation during the early stages of plant development may enhance normal growth under long-term exposure to solar UV. PMID: 23250626
  16. Regeneration of the UVR8 dimer is rapid in intact plants. PMID: 23129206
  17. UVR8 promotes photosynthetic efficiency at elevated levels of UV-B. PMID: 23161229
  18. UVR8-interacting proteins repressor of UV-B photomorphogenesis (RUP)1 and RUP2 mediate UVR8 redimerization independently of COP1 PMID: 23277547
  19. C-terminal region of the UV-B photoreceptor UVR8 initiates signaling through interaction with the COP1 protein. PMID: 22988111
  20. UVR8 plays an important role in mediating the effects of UV-B radiation on pathogen resistance by controlling the expression of the sinapate biosynthetic pathway. PMID: 22447155
  21. crystal structure of UVR8 at 1.8 A resolution, revealing a symmetric homodimer of seven-bladed beta-propeller that is devoid of any external cofactor as the chromophore PMID: 22388820
  22. crystallographic and solution structures of the UVR8 homodimer, together with mutagenesis and far-UV circular dichroism spectroscopy, reveal its mechanisms for UV-B perception and signal transduction PMID: 22323738
  23. UVR8 has in fact in itself the ability to be an ultraviolet-B photoreceptor in plants PMID: 21561100
  24. interaction of the key ultraviolet-B specifc regulator with UVR8 with chromatin in Arabopsis. PMID: 20031919
  25. UV-B stimulates the nuclear accumulation of both a green fluorescent protein (GFP)-UVR8 fusion and native UVR8. PMID: 17720867
  26. UVR8 is involved in controlling aspects of leaf growth and morphogenesis. PMID: 19402876

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Subcellular Location
Nucleus. Cytoplasm, cytosol. Note=UV-B promotes rapid accumulation of UVR8 in the nucleus.
Database Links

KEGG: ath:AT5G63860

STRING: 3702.AT5G63860.1

UniGene: At.49214

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