uvrA Antibody

Code CSB-PA363832XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) uvrA Polyclonal antibody
Uniprot No.
Target Names
uvrA
Alternative Names
uvrA antibody; dinE antibody; b4058 antibody; JW4019 antibody; UvrABC system protein A antibody; UvrA protein antibody; Excinuclease ABC subunit A antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) uvrA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.
Gene References into Functions
  1. The movement of beta-hairpin in the third zinc-binding module of UvrA (Zn3hp) is required for damage-specific binding, UvrB loading and ATPase activities of UvrA. The conformational change of Zn3hp is controlled by ATP hydrolysis at the distal nucleotide binding site. A bi-phasic damage inspection model of UvrA was proposed in which movement of the Zn3hp plays a key role in damage recognition. PMID: 28209516
  2. Data demonstrate that the C-terminal zinc finger of UvrA is required for regulation of damage-specific DNA binding. PMID: 16829526
  3. UvrA is induced after UV irradiation. UvrA levels decrease principally as a result of ClpXP-dependent protein degradation. PMID: 19183285
Subcellular Location
Cytoplasm.
Protein Families
ABC transporter superfamily, UvrA family
Database Links

KEGG: ecj:JW4019

STRING: 316407.85676810

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