uvrB Antibody

Code CSB-PA534351XA01ENP
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) uvrB Polyclonal antibody
Uniprot No.
Target Names
uvrB
Alternative Names
uvrB antibody; EcolC_2864 antibody; UvrABC system protein B antibody; Protein UvrB antibody; Excinuclease ABC subunit B antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Immunogen
Recombinant Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) uvrB protein
Immunogen Species
Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.
Subcellular Location
Cytoplasm.
Protein Families
UvrB family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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