Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) uvrB Polyclonal antibody

Uniprot No.

P0A8F8

Target Names

uvrB

Alternative Names

uvrB antibody; b0779 antibody; JW0762 antibody; UvrABC system protein B antibody; Protein UvrB antibody; Excinuclease ABC subunit B antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) uvrB protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.

Gene References into Functions

ATPase domain, ATP binding domain and/or the DNA binding domain of the UvrB protein are required for DNA repair. PMID: 23058633
study visualized how UvrA and UvrB interacted with DNA individually or together by single- molecule imaging of quantum-dot-labeled proteins PMID: 20227373
we examined the interaction of UvrABC with a DNA substrate containing a single G[8,5-Me]T cross-link and compared it with T[6,4]T (the 6-4 pyrimidine-pyrimidone photoproduct) and the C8 guanine adduct of N-acetyl-2-aminofluorene (AAF) PMID: 16167825
transcription-repair ATP-dependent coupling factor-N reveals a fold that resembles the three N-terminal domains of the repair enzyme UvrB PMID: 16309703
In solution, two UvrB subunits bind to UvrA, most likely as part of a UvrA2B2 complex. The UvrA-induced dimerization of the C-terminal domain 4 regions of UvrB serves to shield these domains from premature UvrC binding. PMID: 17630776
Exploring damage recognition models in prokaryotic nucleotide excision repair with a benzo[a]pyrene-derived lesion in UvrB. PMID: 19681599
found that the N-terminal domain and the unstructured region at the C-terminus of UvrD interact with UvrB. PMID: 19762288

Hide All

Subcellular Location

Cytoplasm.

Protein Families

UvrB family

Database Links

KEGG: ecj:JW0762

STRING: 316385.ECDH10B_0847

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uvrB Antibody

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