Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans vab-1 Polyclonal antibody

Uniprot No.

O61460

Target Names

vab-1

Alternative Names

vab-1 antibody; M03A1.1 antibody; Ephrin receptor 1 antibody; EC 2.7.10.1 antibody; Tyrosine-protein kinase Eph receptor antibody; Variable abnormal protein 1 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans vab-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Receptor for members of the ephrin family. Receptor for major sperm proteins (MSPs), that functions as sperm-sensing checkpoint which inhibits oocyte meiotic maturation and ovulation when sperm are not available for fertilization. Specifically, functions to negatively regulates oocyte maturation and MAPK activation in the absence of MSPs. Required for the MSP-mediated increase in the basal sheath cell contraction rate in somatic cells. Phosphorylates phosphatase daf-18/PTEN which probably promotes daf-18 degradation. By inactivating daf-18, regulates positively insulin-like daf-2 signaling cascade. Involved in interactions between neuronal substrate cells and a migrating epithelial sheet in head epidermis morphogenesis. Also required for cell movements following gastrulation and during ventral closure of the epidermis. Might play a role in spermatheca morphogenesis. Involved in axon guidance of SDQL neurons during neurogenesis.

Gene References into Functions

Overall locomotion speed increased with increasing nicotine concentration in all tested mutant strains except in the vab-1 mutants PMID: 26574927
Analysis of EFN-1(VAB2) expression and tissue-specific requirements is consistent with a model in which VAB-1 acts in amphid neurons, interacting with EFN-1 expressed on surrounding cells. PMID: 23979582
VAB-1/Eph RTK can stop axonal outgrowth by inhibiting filopodia formation at the growth cone by activating Arp2/3 through a VAB-1/NCK-1/WSP-1 complex and by inhibiting UNC-34/Ena activity PMID: 22383893
The germline apoptosis function of VAB-1 impacts specific cell death pathways, and may act in parallel to extracellular signal-regulated kinase MAPK signaling. PMID: 22240896
Bridge formation requires VAB-1 kinase-dependent extension of presumptive bridge cells over scaffold cells toward the ventral midline during body wall closure PMID: 22197242
identified the VAB-1 Eph receptor protein-tyrosine kinase as a major sperm protein receptor PMID: 12533508
Data show that SAX-3/Robo functions with the VAB-1 Eph receptor for gastrulation cleft closure and ventral epidermal enclosure. PMID: 16033794
VAB-1 switches from a negative regulator into a redundant positive regulator of oocyte maturation upon binding to MSP. NMR-1 mediates this switch by controlling UNC-43 CaMKII activation at the oocyte cortex. PMID: 16267094
VAB-1 Eph RTK is required for targeting or limiting axons and neuronal cells to specific regions, perhaps by transducing a repellent or stop cue. PMID: 16386725
This study shows that mutations in ina-1 (alpha-integrin), as well as vab-1 (Eph receptor), and vab-2 (ephrin), display defects in embryonic muscle cell migration. PMID: 18452914
Regulated endocytic trafficking of the VAB-1 MSP/Eph receptor contributes to the control of oocyte meiotic maturation. PMID: 18472420
Hypoxia exerted its [adverse] effect on axon pathfinding at least in part through HIF-1-dependent regulation of the Eph receptor VAB-1. PMID: 18587389

Hide All

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, Ephrin receptor subfamily

Tissue Specificity

During ventral enclosure of the epidermis, expression is seen in clusters of presumptive head neuronal cells and several cells in the tail region. Early larvae show expression in the nerve ring and ventral nerve cord. Strong expression is also seen in the

Database Links

KEGG: cel:CELE_M03A1.1

STRING: 6239.M03A1.1b

UniGene: Cel.5002

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA526454XA01CXY
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

vab-1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background