Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VAL1 Polyclonal antibody

Uniprot No.

Q8W4L5

Target Names

VAL1

Alternative Names

VAL1 antibody; HSI2 antibody; At2g30470 antibody; T6B20.17 antibody; B3 domain-containing transcription repressor VAL1 antibody; Protein HIGH-LEVEL EXPRESSION OF SUGAR-INDUCIBLE 2 antibody; Protein VP1/ABI3-LIKE 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana VAL1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Transcriptional repressor of gene expression involved in embryonic pathways, such as LEC1, ABI3, and FUS3. Repressor of the sugar-inducible genes involved in the seed maturation program in seedlings. Plays an essential role in regulating the transition from seed maturation to seedling growth. Functionally redundant with VAL2/HSL1.

Gene References into Functions

VAL1 crystal structure provides insight into the important implication of VAL1-B3 domain in flowering process. PMID: 29733847
The results suggest that HSI2 recruits MED13 and HDA6 to suppress directly a subset of seed maturation genes post-germination. PMID: 27335347
LEC1, LEC2 , and FUSCA3 transcripts are candidate targets of VAL1, acting through epigenetic and/or transcriptional repression. PMID: 26678037
VAL1 localizes to the nucleation region in vivo, promoting histone deacetylation and FLOWERING LOCUS C transcriptional silencing, and interacts with components of the conserved apoptosis- and splicing-associated protein complex. PMID: 27471304
HSI2 PHD-like domain in the regulation of gene expression involving histone modifications and DNA methylation-mediated epigenetic mechanisms. PMID: 25367506
These results implicate HSI2 as a negative regulator of drought stress response in Arabidopsis. PMID: 24168327
A novel HSI2 mutant allele, hsi2-4, was identified that exhibits constitutively elevated luciferase expression while expression of the endogenous GSTF8 transcript remains unchanged. Microarray analysis indicated that the HSI2 PHD-like domain functions non-redundantly to repress a subset of seed maturation genes. [HSI2] PMID: 22476218
These results indicate that HSI2 and related proteins are B3 domain-EAR motif active transcription repressors. [HSI2] PMID: 15894743

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Subcellular Location

Nucleus.

Tissue Specificity

Expressed in flowers and at lower levels in roots, stems and leaves.

Database Links

KEGG: ath:AT2G30470

STRING: 3702.AT2G30470.1

UniGene: At.27438

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VAL1 Antibody

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