VCS Antibody

Code CSB-PA924790XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VCS Polyclonal antibody
Uniprot No.
Target Names
VCS
Alternative Names
VCS antibody; EDC4 antibody; At3g13300 antibody; MDC11.13 antibody; Enhancer of mRNA-decapping protein 4 antibody; Protein VARICOSE antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana VCS protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
As a component of the decapping complex, involved in the degradation of mRNAs. Essential for postembryonic development, especially during the formation of the shoot (SAM) and root apical meristems. Required for normal patterning of internal tissues of leaves.
Gene References into Functions
  1. ABA-unresponsive subclass I SnRK2s and their downstream effector, VCS, regulate mRNA decay and mRNA accumulation and are required to maintain plant growth under osmotic stress conditions PMID: 28059081
  2. Data suggest that specific motifs, secondary energy, and GC content of transcripts could play a role in their degradation by exoribonuclease4 (xrn4) and varicose (vcs). PMID: 28126845
  3. Seedling lethal phenotypes in dcp1, dcp2 and vcs mutants are caused the disruption of miRNA-mediated gene regulation. PMID: 22614834
  4. DCP2 interacts in vitro and in vivo with DCP1 and VARICOSE (VCS), an Arabidopsis homolog of human Hedls/Ge-1, suggesting that the three proteins operate as a decapping complex. PMID: 17158604
Subcellular Location
Cytoplasm, P-body.
Protein Families
WD repeat EDC4 family
Tissue Specificity
Present in seedlings, roots, hypocotyls, cotyledons, leaves, stems, shoot apex, siliques and flowers. In leaves, particularly present in trichomes, some stomata and vascular tissues.
Database Links

KEGG: ath:AT3G13300

STRING: 3702.AT3G13300.1

UniGene: At.8098

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