VLN3 Antibody

Code CSB-PA529315XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VLN3 Polyclonal antibody
Uniprot No.
Target Names
VLN3
Alternative Names
VLN3 antibody; At3g57410 antibody; F28O9.260 antibody; T8H10.10 antibody; Villin-3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana VLN3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Binds actin and actin filament bundles in a Ca(2+)-insensitive manner, but severs actin filaments in a calcium-dependent manner, regardless of the presence or not of VLN1 (AC O81643). Acts redundantly with VLN2 (AC O81644) to generate thick actin filament bundles, to regulate directional organ growth and in sclerenchyma development.
Gene References into Functions
  1. The headpiece region of VLN3 is required for the bundling of actin filaments. PMID: 22209875
  2. VLN3 binds to and bundles F-Actin in a Ca2+-insensitive manner. It severs actin filaments in a Ca2+-dependent manner. PMID: 20807878
Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Villin/gelsolin family
Tissue Specificity
Expressed in all tissues examined, including root hairs.
Database Links

KEGG: ath:AT3G57410

STRING: 3702.AT3G57410.1

UniGene: At.22370

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