Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VIN3 Polyclonal antibody

Uniprot No.

Q9FIE3

Target Names

VIN3

Alternative Names

VIN3 antibody; At5g57380 antibody; MSF19.4Protein VERNALIZATION INSENSITIVE 3 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana VIN3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Plays a central role in vernalization by mediating the initial transcriptional repression of the homeotic gene FLC, a floral repressor, after a cold treatment. However, due to its transient expression, it cannot maintain repression of FLC, which is then maintained by Polycomb Group complexes containing VRN2 throughout development. Required to deacetylate histones on the FLC promoter. Together with VIL1, required during vernalization for the modifications of FLC and FLM chromatin that are associated with an epigenetically silenced state (e.g. chromatin modifications, histone deacetylation, and trimethylated H3 'Lys-4' H3K4me3 and 'Lys-27' H3K27me3) and with acquisition of competence to flower.

Gene References into Functions

The binding specificity of the PHD-finger domain of VIN3 plays a role in mediating a proper vernalization response in Arabidopsis. PMID: 27999085
VIN3 family of proteins nonredundantly functions to repress different subsets of the FLC gene family during the course of vernalization. PMID: 23417034
The chain of events leading to the expression of VIN3, including changes at the chromatin level, are reported. PMID: 21051939
VIN3 is under a constitutively repressed state, which is transiently relieved from repression only when sufficient cold is provided. PMID: 20671111
During short-term cold exposure, histone H3 acetylation at the transcription start site rapidly increased, implying that it is required for VIN3 induction. PMID: 19825652
VRN2 and VIN3 are part of a large protein complex that can include the PcG proteins FERTILIZATION INDEPENDENT ENDOSPERM, CURLY LEAF, and SWINGER, that is responsible for histone deacetylation at FLC and histone methylation at FLC in vernalized plants. PMID: 16983073
VIN3 is induced in response to hypoxic conditions, but is not induced in response to other stresses that affect redox homeostasis. PMID: 19392705

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Subcellular Location

Nucleus. Nucleus speckle. Note=Probably DNA-associated; interacts directly with the FLC regulatory regions.

Tissue Specificity

Expressed in shoot and root apices. Displays the same pattern of expression as FLC.

Database Links

KEGG: ath:AT5G57380

STRING: 3702.AT5G57380.1

UniGene: At.22442

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VIN3 Antibody

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