Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VIP3 Polyclonal antibody

Uniprot No.

Q9SZQ5

Target Names

VIP3

Alternative Names

VIP3 antibody; SKI8 antibody; At4g29830 antibody; F27B13.70 antibody; WD repeat-containing protein VIP3 antibody; Protein BOUQUET-1 antibody; boq-1 antibody; Protein SKI8 homolog antibody; Protein VERNALIZATION INDEPENDENCE 3 antibody; Protein ZWERGERL antibody; zwg antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana VIP3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of the PAF1 complex (PAF1C) which is involved in histone modifications such as methylation on histone H3 'Lys-4' (H3K4me3). Involved in regulation of flowering time. Required for the expression of the flowering repressor and MADS box gene FLC. Required for histone H3 trimethylation on 'Lys-4' (H3K4me3) and histone dimethylation on 'Lys-36' (H3K36me2) at the FLC locus. Prevents trimethylation on 'Lys-27' (H3K27me3) at the same locus. Not required for meiotic recombination or progression. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C). Required for proper progression of cell differentiation process.

Gene References into Functions

Phyllotaxis regularity depends on the function of VIP proteins, components of the RNA polymerase II-associated factor 1 complex (Paf1c). Divergence angles between successive organs along the stem exhibited increased variance in vip3-1 and vip3-2 compared with the wild type, in two different growth conditions. PMID: 28982682
boq-1 missense mutation enhances meristem formation. PMID: 23134555
phenotypic aspects of VIP3 function in Paf1c and the SKI complex can be separated; we provide evidence that the dual role of SKI8 homologs(VIP3) in Paf1c and the SKI complex appears to depend on the species-specific cellular context. PMID: 22511887
We showed that the Arabidopsis Ski8 was required for normal plant development and growth, suggesting a conserved somatic function, but that it was not required for meiotic recombination or progression [SKI8] PMID: 16716192

Subcellular Location

Nucleus. Cytoplasm.

Database Links

KEGG: ath:AT4G29830

STRING: 3702.AT4G29830.1

UniGene: At.23602

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VIP3 Antibody

Product Details

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Target Background