VIT1 Antibody

Code CSB-PA174646XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VIT1 Polyclonal antibody
Uniprot No.
Target Names
VIT1
Alternative Names
VIT1 antibody; At2g01770 antibody; T8O11.6 antibody; Vacuolar iron transporter 1 antibody; AtVIT1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana VIT1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Vacuolar iron transporter involved in the transfer of iron ions from the cytosol to the vacuole for intracellular iron storage. Involved in regulation of cellular iron homeostasis. Vacuolar iron storage is required for seed embryo and seedling development.
Gene References into Functions
  1. Expression of AtVIT1 in transgenic cassava plants showed a positive correlation with the increase in iron concentration of storage roots. Conversely, young leaves of AtVIT1 transgenic plants exhibit characteristics of iron deficiency. PMID: 26475197
  2. the function of three Arabidopsis proteins with homology to Vacuolar Iron Transporter1 PMID: 25360591
  3. findings provide functional proof that VIT1 mediates iron sequestration into vacuoles of seeds [VIT1] PMID: 17082420
Subcellular Location
Vacuole membrane; Multi-pass membrane protein.
Protein Families
CCC1 family
Tissue Specificity
Highly expressed in developing embryo and seed. Expressed in young seedlings, predominantly in the vasculature.
Database Links

KEGG: ath:AT2G01770

STRING: 3702.AT2G01770.1

UniGene: At.52589

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