VMS1 Antibody

Code CSB-PA189059XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) VMS1 Polyclonal antibody
Uniprot No.
Target Names
VMS1
Alternative Names
VMS1 antibody; YDR049W antibody; Protein VMS1 antibody; VCP/CDC48-associated mitochondrial stress-responsive protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) VMS1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway. Component of an evolutionarily conserved system for ubiquitin-mediated mitochondria-associated protein degradation (MAD), which is necessary to maintain mitochondrial, cellular, and organismal viability.
Gene References into Functions
  1. Data indicate Vms1p as a tRNA hydrolase that releases stalled polypeptides engaged by the ribosome-associated quality control complex (RQC). PMID: 29875445
  2. Cdc48 adaptor Vms1 is a peptidyl-tRNA hydrolase; similar to classical eukaryotic release factor 1, Vms1 activity is dependent on a conserved catalytic glutamine; evolutionary analysis indicates that yeast Vms1 is the founding member of a clade of eukaryotic release factor 1 homologues that are designated the Vms1-like release factor 1 clade PMID: 29632312
  3. Vms1 binds to 60S ribosomes at the mitochondrial surface and antagonizes Rqc2, thereby facilitating import, impeding aggregation, and directing aberrant polypeptides to intra-mitochondrial quality control. PMID: 29107329
  4. Vms1 is preferentially recruited to mitochondria subjected to oxidative stress. PMID: 23468520
  5. the p97/valosin-containing protein (VCP)-interacting motif (VIM) delineates a new family of p97 cofactors PMID: 21896481

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Subcellular Location
Cytoplasm. Mitochondrion. Endoplasmic reticulum membrane; Peripheral membrane protein. Note=translocates from the cytosol to mitochondria upon mitochondrial stress.
Protein Families
ANKZF1/VMS1 family
Database Links

KEGG: sce:YDR049W

STRING: 4932.YDR049W

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