VPS53 Antibody

Datasheet

Code	CSB-PA439636XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VPS53 Polyclonal antibody

Uniprot No.

Q0WQF4

Target Names

VPS53

Alternative Names

VPS53 antibody; HIT1 antibody; At1g50500 antibody; F11F12.15 antibody; F17J6.4 antibody; Vacuolar protein sorting-associated protein 53 A antibody; AtVPS53 antibody; Protein HEAT-INTOLERANT 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana VPS53 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex facilitates tethering as well as SNARE complex assembly at the Golgi. Required for vesicle trafficking involved in plasma membrane protein composition. Probably involved in pollen tube elongation and other polar growth. Confers basal tolerance to long-term heat stress and osmotic stress, by acclimation of the plasma membrane.

Gene References into Functions

The hit1-1 mutation essentially alters vesicle trafficking and results in changes in the plasma membrane components under non-stress conditions. PMID: 21758000
HIT1 may be involved in vesicle trafficking in plant stress responses.[HIT1] PMID: 16408208

Subcellular Location

Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note=Localized in the GARP complex in the Golgi and post-Golgi compartments.

Protein Families

VPS53 family

Tissue Specificity

Present in pollen. Mostly expressed in vegetative tissues, including leaves, siliques, and stems, and flower buds, and, at lower levels, in roots and mature flowers.

Database Links

KEGG: ath:AT1G50500

STRING: 3702.AT1G50500.2

UniGene: At.23430

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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