Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) VPH1 Polyclonal antibody

Uniprot No.

P32563

Target Names

VPH1

Alternative Names

V-ATPase 95 kDa subunit antibody; V-ATPase a 1 subunit antibody; V-type proton ATPase subunit a antibody; vacuolar isoform antibody; Vacuolar pH protein 1 antibody; Vacuolar proton pump a subunit antibody; Vacuolar proton translocating ATPase subunit a 1 antibody; vph1 antibody; VPH1_YEAST antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) VPH1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Subunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in vacuolar V-ATPase complexes. Enzymes containing this subunit have a 4-fold higher ratio of proton transport to ATP hydrolysis than complexes containing the Golgi/endosomal isoform and undergo reversible dissociation of V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Gene References into Functions

we show that the role of Sch9 in regulating ageing is tightly connected with V-ATPase activity and vacuolar acidity. As both Sch9 and the V-ATPase are highly conserved in higher eukaryotes, it will be interesting to further clarify their cooperative action on the cellular processes that influence growth and ageing. PMID: 28604780
Inhibitors of V-ATPase proton transport reveal uncoupling functions of tether linking cytosolic and membrane domains of V0 subunit a (Vph1p). PMID: 22215674
Consequences of loss of Vph1 protein-containing vacuolar ATPases (V-ATPases) for overall cellular pH homeostasis. PMID: 21669878
the large flexibility of R735 might be important for its function in proton translocation in the V-ATPase enzyme PMID: 16257593
osteopetrotic a3 mutations recreated in yeast retain 65-70% of activity with wild type expression of V-ATPase subunits; human a4 dRTA mutation G820R recreated in yeast resulted in inactive but assembled complex in the vacuole membrane PMID: 16840787
Membrane-bound peptides from V-ATPase subunit a do not interact with an indole-type inhibitor. PMID: 18069732

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Subcellular Location

Vacuole membrane; Multi-pass membrane protein.

Protein Families

V-ATPase 116 kDa subunit family

Database Links

KEGG: sce:YOR270C

STRING: 4932.YOR270C

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VPH1 Antibody

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Target Background