VPS15 Antibody

Code CSB-PA178849XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VPS15 Polyclonal antibody
Uniprot No.
Target Names
VPS15
Alternative Names
VPS15 antibody; At4g29380 antibody; F17A13.200 antibody; Serine/threonine-protein kinase VPS15 antibody; EC 2.7.11.1 antibody; Vacuolar protein sorting-associated protein 15 antibody; AtVPS15 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana VPS15 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Serine/threonine-protein kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. Required for pollen development and germination, probably via the modulation of phosphatidylinositol 3-phosphate (PI3P) formation and vacuolar organization.
Gene References into Functions
  1. Data demonstrated that AtVPS15 might interact directly with AtVPS34 and suggested that AtVPS15 is very important for pollen germination, possibly through modulation of the activity of PI3-kinase. PMID: 22361507
  2. Data suggest that AtVPS15 (At4g29380; NP_194667.1) functions in pollen development and germination by regulating PI3P biosynthesis in Arabidopsis. PMID: 21833541
Subcellular Location
Cytoplasm. Golgi apparatus, trans-Golgi network membrane; Lipid-anchor. Endosome membrane; Lipid-anchor.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity
Mainly expressed in anthers, pollen grains and pollen tubes, and, to a lower extent, in other tissues and organs including seedlings, roots, stems, leaves, flowers, pitils and siliques.
Database Links

KEGG: ath:AT4G29380

STRING: 3702.AT4G29380.1

UniGene: At.27874

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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