Vps25 Antibody

Code CSB-PA741957XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Vps25 Polyclonal antibody
Uniprot No.
Target Names
Vps25
Alternative Names
Vps25 antibody; l(2)44Db antibody; CG14750Vacuolar protein-sorting-associated protein 25 antibody; ESCRT-II complex subunit VPS25 antibody; Vacuolar protein sorting 25 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Vps25 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Seems to function as a tumor suppressor by regulating Notch trafficking, hence preventing non-autonomous overproliferation. May be involved in the regulation of autophagy. ESCRT-II interacts with bicoid mRNA, which is required for the anterior localization of bicoid mRNA in the developing egg.
Gene References into Functions
  1. ESCRT-II mutant tissues have deregulated signal transduction pathways, leading to tumorigenesis. PMID: 23418496
  2. The non-cell autonomous induction of Yorkie is due to Notch signaling from vps25 mutant cells. PMID: 22679484
  3. The vps25 protein, a component of the ESCRT machinery that regulates endocytic sorting of signaling receptors, as an unconventional type of Drosophila tumor suppressor. PMID: 16256743
  4. The vps25 protein encodes a component of the ESCRT-II complex, which sorts membrane proteins into multivesicular bodies during endocytic trafficking to the lysosome. PMID: 16256745
Subcellular Location
Cytoplasm. Endosome membrane.
Protein Families
VPS25 family
Database Links

KEGG: dme:Dmel_CG14750

STRING: 7227.FBpp0087774

UniGene: Dm.4897

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