SKD1 Antibody

Datasheet

Code	CSB-PA163483XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SKD1 Polyclonal antibody

Uniprot No.

Q9ZNT0

Target Names

SKD1

Alternative Names

SKD1 antibody; VPS4 antibody; At2g27600 antibody; F10A12.27 antibody; F15K20Protein SUPPRESSOR OF K(+) TRANSPORT GROWTH DEFECT 1 antibody; AtSKD1 antibody; EC 3.6.4.6 antibody; Protein VACUOLAR PROTEIN SORTING 4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SKD1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Involved in the transport of biosynthetic membrane proteins from the prevacuolar/endosomal compartment to the vacuole. Required for multivesicular body (MVB) protein sorting. Catalyzes the ATP-dependent dissociation of class E VPS proteins from endosomal membranes, such as the disassembly of the ESCRT-III complex. May also regulate cell cycle. Required during seed development for the formation of mucilage in seed coat and testa. Involved in the maintenance of Na(+)/K(+) homeostasis under salt stress. Required for cell expansion.

Gene References into Functions

Seeds of the dominant-negative AtSKD1 under control of GS2 promoter display a transparent testa phenotype caused by a lack of proanthocyanidin (PA) and do not possess seed coat mucilage. PMID: 20930567
Dominant-negative AtSKD1 induces vacuolar defects in Arabidopsis trichomes and specifically inhibits vacuolar trafficking. PMID: 20663085

Subcellular Location

Cytoplasm. Nucleus. Endosome, multivesicular body membrane; Peripheral membrane protein. Prevacuolar compartment membrane; Peripheral membrane protein.

Protein Families

AAA ATPase family

Tissue Specificity

Mostly expressed in leaves, to a lower extent in seeds, and barely in roots and flowers (at protein level). Particularly expressed in trichomes.

Database Links

KEGG: ath:AT2G27600

STRING: 3702.AT2G27600.1

UniGene: At.13265

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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