VPS9A Antibody

Code CSB-PA885430XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VPS9A Polyclonal antibody
Uniprot No.
Target Names
VPS9A
Alternative Names
VPS9A antibody; VPS9 antibody; At3g19770 antibody; MMB12.26Vacuolar protein sorting-associated protein 9A antibody; AtVPS9a antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana VPS9A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Functions as guanine nucleotide exchange factor (GEF) for Rab small GTPases. Activates specifically RABF1, RABF2A and RABF2B proteins. Required for early stages of embryogenesis, cytokinesis, embryogenesis, and organ development. Is essential for the establishment or maintenance of the polar localization of the auxin efflux carrier PIN1.
Gene References into Functions
  1. endosome-associated VPS9a, the conserved guanine-nucleotide exchange factor activating Rab5 GTPases, is required for both pre- and postinvasive immunity against a nonadapted powdery mildew fungus. PMID: 28808134
  2. This study investigated the function of the C-terminal region (CTR) by generating truncated versions of VPS9a and found that it was specifically responsible for ARA6 regulation. The CTR was required for oligomerization and correct localization of VPS9a. PMID: 26493488
  3. Data indicate that VPS9a is practically the sole guanine nucleotide exchange factor (GEF) for RAB5s (ARA6) in vegetative developmental stages. PMID: 23893024
  4. Both types of Rab5 members, ARA7 and RHA1 in Arabidopsis, are activated by a single Rab5 GEF, VPS9a. The activity of VPS9a is essential for various plant functions, such as cytokinesis, embryogenesis, and organ development. [VPS9a] PMID: 18055610
Tissue Specificity
Widely expressed.
Database Links

KEGG: ath:AT3G19770

STRING: 3702.AT3G19770.1

UniGene: At.22893

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