Product Details

Full Product Name

Rabbit anti-Simian immunodeficiency virus agm.grivet (isolate AGM gr-1) (SIV-agm.gri) (Simian immunodeficiency virus African green monkey grivet) vpx Polyclonal antibody

Uniprot No.

Q02842

Target Names

vpx

Alternative Names

vpx antibody; Protein Vpx antibody; Viral protein X antibody; X ORF protein antibody

Raised in

Rabbit

Species Reactivity

Simian immunodeficiency virus agm.grivet (isolate AGM gr-1) (SIV-agm.gri) (Simian immunodeficiency virus African green monkey grivet)

Immunogen

Recombinant Simian immunodeficiency virus agm.grivet (isolate AGM gr-1) (SIV-agm.gri) (Simian immunodeficiency virus African green monkey grivet) vpx protein

Immunogen Species

Simian immunodeficiency virus agm.grivet (isolate AGM gr-1) (SIV-agm.gri) (Simian immunodeficiency virus African green monkey grivet)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription.

Gene References into Functions

Data suggest that a critical role of vpx in vivo/in vitro is to promote degradation of SAMHD1 (SAM domain/HD domain protein 1) in memory CD4+ T-lymphocytes, thereby generating high levels of plasma viremia and induction of immunodeficiency. PMID: 25996507
SIV vpx is essential for macrophage infection but not for development of simian AIDS. PMID: 24465411
Inhibition of deoxynucleotide hydrolysis by promoting SAMHD1 degradation is not the only mechanism by which Vpx rescues HIV-1 in mature monocyte-derived dendritic cells from the antiviral state. PMID: 24485168
The authors demonstrate that the Wx4Phix2Phix3APhixH motif in SIVmac Vpx is required for both the Vpx-DCAF1 interaction and/or Vpx-mediated degradation of SAMHD1. PMID: 22776683
However, all the vpx mutant viruses were defective for replication in both human and pigtail monocyte-derived macrophages. PMID: 22531456
Vpx induces proteasomal degradation of SAMHD1 PMID: 21613998
Vpx relieves the inhibition of lentivirus infection in macrophages by loading SAMHD1 onto the CRL4(DCAF1) E3 ubiquitin ligase, leading to highly efficient proteasome-dependent degradation of the protein PMID: 21720370
A single-point mutation in Vpx (H82A) abrogated binding to APOBEC3A and single-round infection of monocytes by HIV-1. PMID: 20178977
Vpx is essential to overcome a block of early infection steps in primary monocytes PMID: 19768115
host cell MAPK signal transduction pathway regulates an early step in SIV infection PMID: 15556948
MacrophageS harbor a potent antiviral restriction; primate lentiviruses have evolved Vpx to counteract this restriction. PMID: 18451984

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Subcellular Location

Virion. Host nucleus.

Protein Families

Lentivirus VPX protein family

Database Links

KEGG: vg:1490006

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vpx Antibody

Product Details

Customer Reviews and Q&A

Target Background