vrk-1 Antibody

Code CSB-PA613924XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans vrk-1 Polyclonal antibody
Uniprot No.
Target Names
vrk-1
Alternative Names
vrk-1 antibody; F28B12.3 antibody; Serine/threonine-protein kinase VRK1 antibody; EC 2.7.11.1 antibody; Vaccinia-related kinase 1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans vrk-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Serine/threonine kinase that phosphorylates baf-1, thus regulating the association of baf-1 with chromatin and nuclear membrane proteins during nuclear envelope formation. May act through the egl-17 signaling pathway. Essential in hermaphrodites for formation of the vulva, uterus, and uterine seam cells and for development and maintenance of the somatic gonad and thus the germ line. Acts to prevent cep-1 from triggering an inappropriate cell cycle arrest, thereby promoting germ cell proliferation. Regulates anchor cell polarity and the timing of anchor cell invasion through the basement membranes separating vulval and somatic gonadal cells during the L3 larval stage.
Gene References into Functions
  1. vrk-1 has a role in early uterine development in Caenorhabditis elegans PMID: 26827901
  2. By coordinating VRK-1- and PP2A-mediated signaling on BAF, Lem4 and LEM-4L (Y55F3BR.8) controls postmitotic nuclear envelope formation in a function conserved from worms to humans. PMID: 22770216
  3. Data show that expression of the fibroblast growth factor-like protein EGL-17 and its receptor EGL-15 is reduced in vrk-1 mutants, suggesting that VRK-1 might act at least partially through activation of FGF signaling. PMID: 19679119
Subcellular Location
Nucleus.
Protein Families
Protein kinase superfamily, CK1 Ser/Thr protein kinase family, VRK subfamily
Tissue Specificity
Present in germ cells at all stages of progression from the mitotic zone to mature oocytes, but not in maturing spermatids (at the protein level). Expressed in the ventral nerve cord and vulva cells.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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