VRP1 Antibody

Code CSB-PA339808XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) VRP1 Polyclonal antibody
Uniprot No.
Target Names
VRP1
Alternative Names
VRP1 antibody; END5 antibody; MDP2 antibody; YLR337C antibody; L8300.13 antibody; Verprolin antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) VRP1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in cytoskeletal organization and cellular growth. May exert its effects on the cytoskeleton directly, or indirectly via proline-binding proteins (e.g. profilin) or proteins possessing SH3 domains.
Gene References into Functions
  1. These studies provide evidence that WASP (Las17) and WIP (Vrp1) play central roles in establishment of a robust multivalent Src homology 3 domain-proline-rich motif network in vivo, giving Actin-related protein 2/3 complex assembly onset at endocytic sites a switch-like behavior. PMID: 28813247
  2. Vrp1 protin is responsible for temperature sensitivity and plays a role in calcineurin activation and inhibition. PMID: 25614218
  3. Findings highlight the role of Las17p binding for Vrp1p membrane association, suggest general membrane association may be more important than specific targeting to the cortical actin cytoskeleton for Vrp1p. function in endocytosis and cell growth. PMID: 17635585
  4. Results identify an N-terminal fragment of Las17p able to interact with Vrp1p but not Arp2/3, and suggest that LAs17 interaction with Vrp1 is more important than interaction with Arp2/3 for polarized actin assembly and endocytosis in yeast. PMID: 19272406
Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Verprolin family
Database Links

KEGG: sce:YLR337C

STRING: 4932.YLR337C

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