vsr Antibody

Code CSB-PA357594XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) vsr Polyclonal antibody
Uniprot No.
Target Names
vsr
Alternative Names
vsr antibody; b1960 antibody; JW1943Very short patch repair protein antibody; EC 3.1.-.- antibody; DNA mismatch endonuclease antibody; V.EcoKDcm antibody; Vsr mismatch endonuclease antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) vsr protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Deamination of 5-methylcytosine in DNA results in T/G mismatches. If unrepaired, these mismatches can lead to C-to-C transition mutations. The very short patch (VSP) repair process in E.coli counteracts the mutagenic process by repairing the mismatches in favor of the G-containing strand. This enzyme is an endonuclease that nicks double-stranded DNA within the sequence CT(AT)GN or NT(AT)GG next to the thymidine residue that is mismatched to 2'-deoxyguanosine. The incision is mismatch-dependent and strand-specific.
Gene References into Functions
  1. Authors propose that lesions formed upon aminoglycoside treatment are preferentially repaired by Vsr without SOS induction in Escherichia coli and by mismatch repair when very short patch mismatch repair is impaired. PMID: 24946128
  2. unusual patterns of base pair stability in the Dcm target sequence can explain how the Vsr endonuclease specifically recognizes the mismatched Dcm target sequence and intercalates into the DNA PMID: 20541519
  3. Vsr carries out a hydrolytic reaction with inversion of stereochemistry at phosphorus, compatible with an in-line attack of water and a pentacovalent transition state with trigonal bipyramidal geometry PMID: 16188275
  4. MutL recruits the Vsr and MutH to sites of DNA damage. PMID: 19376855
  5. Results suggest a mobile MutS-MutL complex in DNA mismatch repair signalling, that leaves the DNA mismatch prior to, or at the time of, activation of downstream effector molecules such as Vsr. PMID: 19474347

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Protein Families
Vsr family
Database Links
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