WAG2 Antibody

Code CSB-PA201861XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) WAG2 Polyclonal antibody
Uniprot No.
Target Names
WAG2
Alternative Names
WAG2 antibody; At3g14370 antibody; MLN21.15Serine/threonine-protein kinase WAG2 antibody; EC 2.7.11.1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana WAG2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Serine/threonine-protein kinase involved in the regulation of auxin signaling. Acts as a positive regulator of cellular auxin efflux and regulates organ development by enhancing PIN-mediated polar auxin transport. Phosphorylates conserved serine residues in the PIN auxin efflux carriers. Phosphorylation of PIN proteins is required and sufficient for apical-basal PIN polarity that enables directional intercellular auxin fluxes, which mediate differential growth, tissue patterning and organogenesis. Acts as suppressors of root waving.
Gene References into Functions
  1. WAG2 is necessary for phytochrome-mediated enhancement of hypocotyl phototropism in Arabidopsis. PMID: 25281709
  2. WAG2 is a crucial transcription target during apical hook opening downstream from GA signaling PMID: 22992959
  3. identified the PID homologs WAG1 and WAG2 as new PIN polarity regulators. PMID: 20823065
  4. wag1 and wag2 are protein kinases that negatively regulate root waving in Arabidopsis. [wag2] PMID: 16460509
Subcellular Location
Cytoplasm, cytosol. Note=Targeted to the cell periphery.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity
Expressed in root tips, lateral root primordia and emerging true leaf primordia.
Database Links

KEGG: ath:AT3G14370

STRING: 3702.AT3G14370.1

UniGene: At.39249

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