ddl-1 Antibody

Code CSB-PA522324XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans ddl-1 Polyclonal antibody
Uniprot No.
Target Names
ddl-1
Alternative Names
ddl-1 antibody; F59E12.10WASH complex subunit homolog 3 antibody; Daf-16-dependent longevity protein 1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans ddl-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Acts as a component of the DHIC (ddl-1-containing hsf-1 inhibitory complex) which modulates lifespan by sequestering the heat-shock transcription factor hsf-1 to negatively regulate its binding to DNA and its transcriptional activity.
Gene References into Functions
  1. Study shows that the proteins DDL-1 and DDL-2, previously implicated in life span extension, modulate HSF-1 activity by forming an inhibitory heterocomplex with HSF-1 and that formation of this complex is regulated by Insulin/IGF-1-like signaling. PMID: 22265419
Protein Families
CCDC53 family
Tissue Specificity
Expressed in pharynx, intestine, body wall muscles, vulva muscles, spermatheca, and several head and tail neurons.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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