WAT1 Antibody

Code CSB-PA213632XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) WAT1 Polyclonal antibody
Uniprot No.
Target Names
WAT1
Alternative Names
WAT1 antibody; At1g75500 antibody; F10A5.28 antibody; F1B16.19Protein WALLS ARE THIN 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana WAT1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for secondary wall formation in fibers, especially in short days conditions. Promotes indole metabolism and transport (e.g. tryptophan, neoglucobrassicin and auxin (indole-3-acetic acid)). May prevent salicylic-acid (SA) accumulation.
Gene References into Functions
  1. The role of WAT1 in salicylic acid and tryptophan and subsequent resistance to Ralstonia solanacearum infection was studied. PMID: 22978675
  2. Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis. PMID: 24129639
  3. These results suggest that free auxin concentration is not responsible for the partial light-regulated complementation of wat1-mediated phenotypes. PMID: 20935503
Subcellular Location
Vacuole membrane; Multi-pass membrane protein. Note=Also detected in transvacuolar strands.
Protein Families
Drug/metabolite transporter (DMT) superfamily, Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family
Tissue Specificity
Mostly expressed in stems and hypocotyls, also present in seedlings, root, leaves, flowers and siliques. Ubiquitous, mostly expressed in vascular tissues and secondary wall-forming cells, including developing xylem vessels and fibers.
Database Links

KEGG: ath:AT1G75500

STRING: 3702.AT1G75500.1

UniGene: At.21884

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