WDR5A Antibody

Code CSB-PA556410XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) WDR5A Polyclonal antibody
Uniprot No.
Target Names
WDR5A
Alternative Names
WDR5A antibody; At3g49660 antibody; T16K5.10COMPASS-like H3K4 histone methylase component WDR5A antibody; AtWDR5A antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana WDR5A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Forms multiple COMPASS-like complexes involved in histone methylation by interacting with different histone H3 'Lys-4' methyltransferases such as ATX1, SDG14 or SDG16. Binds to target loci chromatin, increasing H3K4 trimethylation and causing activation of the gene. Upregulates FLC and MAF4 expression to delay flowering. Present at the promoters and at the transcription start sites (TSS) regions of WRKY70 and LTP7; this occupancy is ATX1-dependent. Involved in the transition from transcription initiation to transcription elongation.
Gene References into Functions
  1. WDR5a functions as a novel factor to modulate nitric oxide synthase-like activity for changes of nitric oxide accumulation and stomatal closure in drought stress tolerance. PMID: 26825291
  2. WDR5a directly interacts with an H3K4 methyltransferase, ARABIDOPSIS TRITHORAX1. PMID: 19567704
Subcellular Location
Nucleus.
Tissue Specificity
Strongly expressed in developing embryos and endosperms. Expressed in shoot and root apical regions, and in vasculature.
Database Links

KEGG: ath:AT3G49660

STRING: 3702.AT3G49660.1

UniGene: At.35578

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