wecA Antibody

Datasheet

Code	CSB-PA364761XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) wecA Polyclonal antibody

Uniprot No.

P0AC78

Target Names

wecA

Alternative Names

wecA antibody; rfe antibody; b3784 antibody; JW3758Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase antibody; EC 2.7.8.33 antibody; UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase antibody; Undecaprenyl-phosphate GlcNAc-1-phosphate transferase antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) wecA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the transfer of the GlcNAc-1-phosphate moiety from UDP-GlcNAc onto the carrier lipid undecaprenyl phosphate (C55-P), yielding GlcNAc-pyrophosphoryl-undecaprenyl (GlcNAc-PP-C55). It is the first lipid-linked intermediate involved in enterobacterial common antigen (ECA) synthesis, and an acceptor for the addition of subsequent sugars to complete the biosynthesis of O-antigen lipopolysaccharide (LPS) in many E.coli O types. The apparent affinity of WecA for the polyisoprenyl phosphate substrates increases with the polyisoprenyl chain length. WecA is unable to utilize dolichyl phosphate (Dol-P).

Gene References into Functions

characterization of the functional roles of the conserved aspartic acids of WecA PMID: 17237164
An enzymatic assay of WecA was developed, and the kinetic parameters as well as the effects of pH, salts, cations, detergents, and temperature on the enzyme activity were determined. A minimal length of 35 carbons was required for the lipid substrate. PMID: 18723618

Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

Glycosyltransferase 4 family, WecA subfamily

Database Links

KEGG: ecj:JW3758

STRING: 316385.ECDH10B_3973

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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wecA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background