WEE1 Antibody

Code CSB-PA811852XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) WEE1 Polyclonal antibody
Uniprot No.
Target Names
WEE1
Alternative Names
WEE1 antibody; At1g02970 antibody; F22D16.3 antibody; Wee1-like protein kinase antibody; EC 2.7.10.2 antibody; Wee1-At antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana WEE1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Cell cycle regulatory kinase that is not rate-limiting for cycle progression under normal growth conditions. Transcriptionally activated upon DNA stress or damage in an ATR- or ATM-dependent manner. Once activated, inhibits plant growth by arresting dividing cells in the G2 phase before proceeding into mitosis. Down-regulates CDKA-1 and CDKD-2 by tyrosine phosphorylation. May target principally CDKA-1.
Gene References into Functions
  1. the identification of a second member of the RNase H2 complex, RNase H2B, being able to complement the root growth phenotype of WEE1(KO) plants, is reported. PMID: 25875879
  2. The plant WEE1 kinase acquired an indirect developmental function that is important for meristem maintenance upon replication stress. PMID: 21498679
  3. The WEE1 gene is not rate-limiting for cell cycle progression under normal growth conditions but is a critical target of the ATR-ATM signaling cascades that inhibit the cell cycle upon activation of the DNA integrity checkpoints. PMID: 17209125
Subcellular Location
Nucleus.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily
Tissue Specificity
Expressed in shoot apex, vasculatures tissues of roots and leaves, and developing flowers.
Database Links

KEGG: ath:AT1G02970

STRING: 3702.AT1G02970.1

UniGene: At.42570

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