Wee1 Antibody

Code CSB-PA026084XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Wee1 Polyclonal antibody
Uniprot No.
Target Names
Wee1
Alternative Names
Wee1 antibody; wee antibody; CG4488Wee1-like protein kinase antibody; Dwee1 antibody; EC 2.7.10.2 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Wee1 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as a negative regulator of entry into mitosis (G2 to M transition). This kinase specifically phosphorylates and inactivates cyclin B1-complexed CDC2.
Gene References into Functions
  1. Relationships between DNA replication, chromosome condensation, and anaphase entry are mediated by the cell cycle kinases Grp (Chk1) and dWee1 (Wee1). PMID: 22262459
  2. Phosphorylation of the KLP61F catalytic domain by dWee1 is important for the motor's function. PMID: 19800237
  3. Wee1 kinases can regulate mitotic entry in vivo during metazoan development even in cycles that lack a G2 phase. PMID: 15589158
  4. Drosophila Wee1 interacts with members of the gammaTURC and is required for proper mitotic-spindle morphogenesis and positioning. PMID: 16139207
Subcellular Location
Nucleus.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily
Tissue Specificity
Expressed in embryos; expression remains high in the proliferating cells of the central nervous system well after cells in the rest of the embryo have ceased dividing.
Database Links

KEGG: dme:Dmel_CG4488

STRING: 7227.FBpp0304607

UniGene: Dm.3106

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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