whiB1 Antibody

Datasheet

Code	CSB-PA617584XA01FSG
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) whiB1 Polyclonal antibody

Uniprot No.

P9WF43

Target Names

whiB1

Alternative Names

whiB1 antibody; Rv3219 antibody; Transcriptional regulator WhiB1 antibody

Raised in

Rabbit

Species Reactivity

Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Immunogen

Recombinant Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) whiB1 protein

Immunogen Species

Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Acts as a transcriptional repressor, inhibiting expression in vitro. Probably redox-responsive. The apo- but not holo-form binds to its own promoter as well as that of groEL2. Oxidized apo-form and nitrosylated holo-form also bind DNA. The apo-form has been shown to act as a protein disulfide reductase, but also not to act as a protein disulfide reductase.

Gene References into Functions

NMR structural model of WhiB1 reveals that Wbl proteins are four-helix bundles with a core of three alpha-helices held together by a [4Fe-4S] cluster. The WhiB1 structure suggests that loss of the iron-sulfur cluster (by nitrosylation) permits positively charged residues in the C-terminal helix to engage in DNA binding, triggering a major reprogramming of gene expression that includes components of the ESX-1 secretion ... PMID: 29273788
we were able to unambiguously identify WhiB1, an essential transcriptional repressor capable of auto-repression, as a substrate of the mycobacterial Clp protease. PMID: 24603869
Analysis of the C-terminal region of WhiB1 revealed the crucial roles played by a predicted beta-turn and two conserved positively-charged motifs in facilitating DNA-binding, but not iron-sulfur cluster acquisition. PMID: 22792304
Data suggest whiB1 (with [4Fe-4S] cluster) is activated in presence of nitric oxide allowing whiB1 to bind to DNA (at region overlapping core promoter elements). PMID: 20929442

Subcellular Location

Cytoplasm.

Protein Families

WhiB family

Database Links

KEGG: mtu:Rv3219

STRING: 83332.Rv3219

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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