shf Antibody

Datasheet

Code	CSB-PA893839XA01DLU
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Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) shf Polyclonal antibody

Uniprot No.

Q9W3W5

Target Names

shf

Alternative Names

shf antibody; CG3135 antibody; Protein shifted antibody; WIF-1-like protein antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) shf protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Required for normal accumulation and movement of lipid-modified hedgehog (hh) morphogen. May act by stabilizing the interaction between heparan sulfate proteoglycans (HSPGs) and hh, HSPGs being required for diffusion of hh morphogen. Not involved in wingless (wg) morphogen movement, suggesting that it may provide HSPG specificity for Hh.

Gene References into Functions

We show that production of this PGC attractant depends upon shf as well as a second hh pathway gene g gamma1. PMID: 24068944
study concludes that the WIF domain is responsible for the divergence between Drosophila Shf and human Wif-1, channeling the recognition either toward Hh or Wg signal, whereas the EGF repeats give the protein the ability to interact with glypicans PMID: 22951645
Shf is the ortholog of the human Wnt inhibitory factor (WIF), a secreted antagonist of the Wingless pathway. Shf is required for Hh stability and for lipid-modified Hh diffusion. PMID: 15691765
Shifted is a secreted protein that acts over a long distance and is required for the normal accumulation of Hh protein and its movement in the wing. PMID: 15691766

Subcellular Location

Secreted, extracellular space, extracellular matrix. Note=Colocalizes with HSPG.

Tissue Specificity

At the blastoderm stage, it is ubiquitously expressed. As embryogenesis continues, it is expressed in the epidermis and central nervous system, this expression being segmentally modulated. Also highly expressed at the foregut and hindgut throughout embryo

Database Links

KEGG: dme:Dmel_CG3135

STRING: 7227.FBpp0070939

UniGene: Dm.58

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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shf Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background