Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans mig-14 Polyclonal antibody

Uniprot No.

Q7YWX7

Target Names

mig-14

Alternative Names

mig-14 antibody; mom-3 antibody; R06B9.6 antibody; Protein wntless homolog antibody; Abnormal cell migration protein 14 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans mig-14 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Probable sorting receptor which regulates endocytosis and secretion of the wnt ligand egl-20. Recycling of mig-14 from the plasma membrane to the Golgi apparatus by the retromer complex is essential for its function. Its endosomal trafficking is regulated by its association with sorting nexin snx-3 on early endosomes and the mtm-6/mtm-9 myotubularin complex. Required in embryonic development for endoderm specification and the correct positioning and orientation of the mitotic spindles and division planes in blastomere cells. Functions during vulval development, playing a role in vulval precursor cell fate specification. During development, specifically regulates the migration of HSN neurons, the left Q neuroblast (QL) and its descendants and the distal tip cells of the gonads. Positioning of Q neuroblasts may be both dependent and independent of hox gene mab-5. Involved in establishing ALM and PLM neuronal cell polarity.

Gene References into Functions

The authors conclude that regulation of endosomal trafficking by the MTM-6/MTM-9 myotubularin complex is required for the retromer-dependent recycling of MIG-14/Wls and Wnt secretion. PMID: 21076391
MIG-14, the C. elegans homolog of the Wnt-secretion factor Wntless acts in these cells to control Wnt function PMID: 18160346
Recycling of MIG-14/Wint proteins is part of a trafficking pathway that retrieves MIG-14/Wint proteins from the plasma membrane PMID: 18160347

Subcellular Location

Cell membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Basal cell membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein.

Protein Families

Wntless family

Tissue Specificity

Expressed in the tail hypodermis, stomatointestinal muscle, the mesoblast cell M and its descendants, CAN neurons, the developing vulva, the pharynx and the pharyngeal intestinal valve.

Database Links

KEGG: cel:CELE_R06B9.6

STRING: 6239.R06B9.6

UniGene: Cel.7379

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mig-14 Antibody

Product Details

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Target Background