cwn-1 Antibody

Code CSB-PA339509XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans cwn-1 Polyclonal antibody
Uniprot No.
Target Names
cwn-1
Alternative Names
cwn-1 antibody; wnt-1 antibody; K10B4.6 antibody; Protein Wnt-1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans cwn-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Binds receptor tyrosine kinase cam-1. Together with Wnt ligand cwn-2, regulates the migration of CAN, ALM, BDU and HSN neurons during embryogenesis, the migration of QL and QR neuroblast descendants during larval development, and polarity of ALM neurons. Also acts with the Wnt ligand egl-20 to direct HSN neuron migration. Acts through the Wnt receptor cfz-2 to direct ALM migration. Also plays a role in axon growth and guidance in HSN and male CP neurons. In addition, together with Wnt ligand cwn-2, negatively regulates developmental neurite pruning of AIM neurons probably by acting as a ligand for receptor tyrosine kinase cam-1. Probably by activating the Wnt/Frizzled pathway, may regulate vulva development. May act redundantly with other Wnt ligands such as cwn-2 and mom-2 to control seam cell polarity.
Gene References into Functions
  1. Here, too, we found that four of five Wnt receptors can influence P7.p orientation, suggesting that a surprising amount of functional redundancy exists in Wnt signaling during C. elegans vulval induction. PMID: 16930586
  2. CAM-1 interacts with and inhibits Egl-20, CWN-1 and MOM-2 during vulva morphogenesis. PMID: 17942487
  3. C. elegans Wnt homolog, cwn-1, functions to regulate asymmetric division of the ABpl/rpppa blast cell. Cwn-1 does not act alone in this process, and it functions with another redundant ligand that appears not to be a Wnt. PMID: 19389360
Subcellular Location
Secreted, extracellular space, extracellular matrix. Cytoplasm. Cell membrane; Peripheral membrane protein.
Protein Families
Wnt family
Tissue Specificity
Expressed in intestine, some head neurons and ventral nerve cord and pharyngeal neurons. Expressed in the tail and weakly expressed in the vulva and body wall muscles. Expressed highly in posterior dorsal and ventral muscle cells.
Database Links

KEGG: cel:CELE_K10B4.6

STRING: 6239.K10B4.6a

UniGene: Cel.18277

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