wnt4a Antibody

Code CSB-PA343319XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) wnt4a Polyclonal antibody
Uniprot No.
Target Names
wnt4a
Alternative Names
wnt4a antibody; wnt-4 antibody; wnt4 antibody; Protein Wnt-4a antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) wnt4a protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ligand for members of the frizzled family of seven transmembrane receptors. Plays an important role in embryonic development.
Gene References into Functions
  1. Fox1 activation of Wnt4a in the ectoderm signals the epithelial stabilization of pouch-forming cells during late-stage of pouch morphogenesis. PMID: 29932895
  2. Data show that in vivo, wnt11r and wnt4a initiate MuSK translocation from muscle membranes to recycling endosomes and that this transition is crucial for AChR accumulation at future synaptic sites. PMID: 22318632
  3. Data show that injury-dependent induction of Ascl1a suppressed expression of the Wnt signaling inhibitor, Dkk, and induced expression of the Wnt ligand, Wnt4a. PMID: 21911394
  4. Findings provide the first convincing line of evidence that EAF and Wnt4 form an auto-regulatory negative feedback loop in vivo. PMID: 20161747
  5. three Wnt noncanonical ligands wnt4a, silberblick/wnt11, and wnt11-related regulate the process of convergence of endoderm and organ precursors toward the embryonic midline by acting in a largely redundant way PMID: 15630025

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Wnt family
Tissue Specificity
Caudal forebrain and neural keel, the floor plate, the gill slit and the developing pronephros.
Database Links

KEGG: dre:30123

STRING: 7955.ENSDARP00000095904

UniGene: Dr.385

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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