WOX5 Antibody

Code CSB-PA814116XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) WOX5 Polyclonal antibody
Uniprot No.
Target Names
WOX5
Alternative Names
WOX5 antibody; At3g11260 antibody; F11B9.18 antibody; WUSCHEL-related homeobox 5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana WOX5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor, which may be involved in the specification and maintenance of the stem cells (QC cells) in the root apical meristem (RAM).
Gene References into Functions
  1. the expression switch from WOX11/12 to WOX5/7 is critical for initiation of the root primordium during de novo root organogenesis. PMID: 27784768
  2. WOX5 plays a role in root stem cell niche maintenance. PMID: 26440429
  3. WOX5 signals from the quiescent centre to promote columella stem cell (CSC) fate, while CLE40 is secreted from the differentiated columella cells to promote differentiation. PMID: 26019259
  4. ROW1 is essential for quiescent centre maintenance and for stem cell niche development through the repression of WOX5 in the proximal meristem. PMID: 25631790
  5. Results show that ACR4 phosphorylates WOX5 through four phosphorylation sites within the WOX5 protein sequence. PMID: 25756623
  6. WOX5 restrains cell division in the cells of the quiescent center, thereby establishing quiescence. In contrast, WOX5 and CYCD3;3/CYCD1;1 both promote cell proliferation in the nascent columella. PMID: 25127220
  7. WOX5-IAA17 feedback circuit is essential for the maintenance of auxin gradient in the root tip. PMID: 23939433
  8. The MKO1 gene plays an important role in the maintenance of the root apical meristem proliferative capacity and indeterminate root growth, which apparently acts independently of the SCR/SHR and WOX5 regulatory pathways. PMID: 21744091

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Subcellular Location
Nucleus.
Protein Families
WUS homeobox family
Tissue Specificity
Specifically expressed in the central cells of a quiescent center (QC) of the root.
Database Links

KEGG: ath:AT3G11260

STRING: 3702.AT3G11260.1

UniGene: At.39787

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